【摘要】 目的探讨IFIT1(interferon-induced protein with tetratricopetide repeats 1)在放射损伤与感染应激中表达的特点和意义。方法采用5μg/mL的脂多糖(LPS)刺激Raw264.7、3T3和10T1/2细胞株5 h后,收集细胞,提取RNA,行RT-PCR观察IFIT1的表达情况,同时设定空白对照组。另外,将20只C57/BL6小鼠随机分为4组,采用12 Gray60Co一次性全身照射,分别于0 h、1 h、4 h和12 h脱颈处死取肝组织,用Western blot测定肝组织IFIT1表达变化。结果 Raw264.7、3T3和10T1/2细胞株经LPS刺激5 h后,均能诱导IFIT1表达,空白对照组表达为阴性。C57/BL6小鼠放射性损伤后,1 h IFIT1/Actin开始增高,12 h达最高,呈上升趋势,差异有极显著性统计学意义(P<0.01)。结论 LPS可刺激多种细胞株表达IFIT1,提示IFIT1可能参与创伤后毒血症的发生和发展;放射损伤早期能引起小鼠肝组织IFIT1的显著增高。更多还原

【Abstract】 Objective To explore characteristics and significance of interferon-induced protein with tetratricopetide repeats 1 expressed in radiation injury and infection stress. Methods RNA was extracted from Raw264. 7 cell,3T3 cell and 10T1 /2 cell after 5 hours stimulated with 5 μg / mL LPS. At the same time,to set up normal control group( untreated by LPS),and RNA of IFIT1 was detected by RT-PCR. Totally 20 C57 / BL6 mice were randomly divided into 4 groups,namely 0 h group,1 h group,4 h group and 12 h group. The mice were given 12Gray60 Co full-body exposure once,then liver IFIT1 was detected by western blot. Results Stimulated with LPS for 5 hours,IFIT1 was induced expression in Raw264. 7 cell,3T3 cell and 10T1 /2 cell. The expression of normal control group was negative. The level of IFIT1 / Actin increased significantly 1 hour after radiation injury,and it reached the peak 12 hours after radiation injury( P < 0. 01). Conclusion LPS can stimulated a variety of cell lines expressed IFIT1,prompting that IFIT1 may participate in the occurrence and development of post-traumatic toxemia. IFIT1 of liver tissue increased significantly during the early stage in radiation mice.更多还原