【摘要】 长链非编码RNA(lncRNA)在疾病的发生发展过程中发挥着重要的作用.LncRNA的异常表达与许多肿瘤的发生发展密切相关.本研究通过分析肿瘤基因图集(TCGA)数据库首次鉴定出了在前列腺癌中高表达的长链非编码RNA LINC01126,并在前列腺癌样本和前列腺癌细胞系中得到了验证.通过进一步分析发现,LINC01126的表达量在肿瘤中显著上调(419例前列腺癌组织样本和52例癌旁组织样本,P<0.001),LINC01126表达与临床病理分级的相关性分析中,相比于癌旁组织,Gleason 8样本和Gleason 9样本中的LINC01126表达水平显著增加(52例癌旁组织样本;Gleason 8,n=44,P<0.001;Gleason 9,n=71,P<0.001).进一步的分析结果表明,LINC01126能够较好地指示前列腺癌的Gleason分级.相比于Gleason 6样本,Gleason 8样本和Gleason 9样本中的LINC01126表达水平上调(Gleason 6,n=30;Gleason 8,n=44,P<0.01;Gleason 9,n=71,P<0.05).同样,相比于Gleason 7样本,Gleason 8样本和Gleason 9样本中的LINC01126表达水平也显著增加(Gleason 7,n=193;Gleason 8,n=44,P<0.001;Gleason 9,n=71,P<0.001).LINC01126的表达水平与前列腺癌病理分期的相关性结果提示,LINC01126在发生转移的前列腺癌中的表达量更高,差异显著(T2a,T2b,T2c样本共144例,T3a,T3b,T4样本共194例,P<0.01).另外,还分析了与LINC01126共表达或相反表达的mRNA,作为其可能的调控基因,及其这些基因的生物学进程和相关的信号通路,表明LINC01126与前列腺癌发生发展具有潜在的生物学关联,有望成为前列腺癌的生物标志物.更多还原

【Abstract】 Long non-coding RNA(lncRNA) plays an important role in the development of various diseases. Abnormal expression of lncRNAs is closely associated with tumorigenesis. In this study, we identified a long non-coding RNA, LINC01126, which was over-expressed in prostate cancer. LINC01126 was found to be up-regulated in prostate cancer(419 samples, 52 controls, P < 0.001) by analyzing the Cancer Genome Atlas database. Compared with that in normal tissues, LINC01126 expression was up-regulated in Gleason 8 and Gleason 9 samples(52 control; Gleason 8, n = 44, P < 0.001; Gleason 9, n = 71, P < 0.001). Further analysis showed that LINC01126 was a significant indicator of prostate Gleason classification. LINC01126 expression increased in Gleason 8 and 9 samples compared with that in Gleason 6(Gleason 6, n = 30; Gleason 8, n = 44, P < 0.01; Gleason 9, n = 71, P < 0.05) and Gleason 7 samples(Gleason 7, n = 193; Gleason 8, n = 44, P < 0.001; Gleason 9, n = 71, P < 0.001). Additionally, our results showed that LINC01126 was highly expressed in metastatic prostate cancer(144 T2a, T2b, and T2c samples, 194 T3a, T3b, and T4 samples, P < 0.01). We also identified genes that were negatively or positively regulated by LINC01126 as well as their roles in biological processes and related pathways. In conclusion, our results suggest that LINC01126 is a new molecular biomarker for the diagnosis of prostate cancer.更多还原