【摘要】 目的了解滇西地区室内鼠形动物巴尔通体感染状况。方法在滇西地区8个州(市)抽取10个县(市、区)40个自然村,随机抽取800户家庭(每村20户),进行室内捕鼠,采集鼠脾脏标本并提取基因组DNA,运用聚合酶链反应(PCR)扩增巴尔通体枸橼酸合酶(glt A)基因,阳性者进行测序分析。结果捕获9种421只鼠形动物,采集脏器样本404份,PCR扩增阳性64份,阳性率为15.84%。9种鼠形动物中仅黄胸鼠和褐家鼠检出阳性,阳性率分别为23.05%(62/269)和8.70%(2/23)。10个县(市、区)中6个检出阳性。基因分析表明携带的巴尔通体至少存在6种基因型:B.tribocorum、B.queenslandensis、B.elizabethae、B.rochalimae及2个可能的新型。结论滇西地区室内鼠形动物感染巴尔通体,且呈现基因多样性特征,部分县(市)阳性率较高,需加强监测和预防控制。更多还原

【Abstract】 Objective To understand the Bartonella infection status in small rodents in households in western area of Yunnan province. Methods A total of 800 households were randomly selected from 40 natural villages of 10 counties in western Yunnan. Small rodents were captured for 3 consecutive nights in each household. The liver and spleen specimen of small rodents were collected for DNA extraction. The glt A gene of Bartonella was amplified with PCR and gene-sequencing test was conducted for PCR amplification products. Results A total of 421 small rodents,belonging to 9 species,were captured,and404 specimens were collected and tested with PCR. The Bartonella positive rate was 15. 84%( 64 /404). Of 9 species,the part of specimens from Rattus tanezumi and Rattus norvegicus were positive for Bartonella and the positive rate was 23. 05%( 62 /269) and 8. 70%( 2 /23),respectively. Among 10 counties,the specimens collected from 6 counties were positive.Gene analysis showed that there were 6 gene types of Bartonella infection, which included B. tribocorum,B. queenslandensis,B. elizabethae and B. rochalimae,and 2 possible newgene types were found. Conclusion Bartonella infection was detected in small rodents in households in western Yunnan and the gene diversity was observed. Bartonella infection rate in some counties was high, indicating that the surveillance and control / prevention measures need to be strengthened.更多还原