【摘要】 目的该文采用高效液相色谱技术,对急性缺血性卒中患者红细胞膜磷脂含量分析,探讨红细胞膜磷脂水平在急性缺血性卒中发生、发展中的作用机制。方法采用流行病学横断面调查方法,自2015年7—10月筛选病例组人群22例,健康对照组人群24名,分离红细胞膜,用HPLC,XBP-Silica柱分离,测定两组人群红细胞膜磷脂含量,数据采用SPSS 20.0统计学软件分析。结果 5种磷脂中脂酰胆碱(PC)、磷脂酰乙醇胺(PE)、磷脂酰丝氨酸(PS)3种磷脂病例组含量(0.3796±0.1387)、(0.4498±0.1786)、(0.3301±0.0327)与对照组(0.4897±0.0154)、(0.5523±0.0107)、(0.6351±0.0599)数值差异有统计学意义(P<0.05),磷脂酰肌醇(PI)和神经鞘磷脂(SM)差异无统计学意义(P>0.05)。结论红细胞膜磷脂水平参与影响了急性缺血性卒中的发生发展过程,可能是急性缺血性卒中发生发展的一个诱导机制。更多还原

【Abstract】 Objective Using HPLCtechnique, the red cell membrane phospholipids content analysis in patients with acute ischemic stroke and explore the red cell membrane phospholipids level in acute ischemic stroke in the occurrence and development mechanism. Methods Using cross-sectional survey methods, from July to October 2015 were 22 groups, healthy control group 24, with HPLC of erythrocyte membrane separation, XBP-Silica column separation and determination of phospholipid content of erythrocyte membrane in two groups, data using SPSS 20.0 statistical analysis software. Results 5 kinds of phospholipid phosphatidylcholine(PC), phosphatidylethanolamine(PE), phosphatidylserine(PS) three cases(0.3796± 0.1387),(0.4498 ±0.1786),(0.3301 ± 0.0327) and control group(0.4897±0.0154),(0.5523±0.0107),( 0.6351±0.0599) value was statistically significant the difference(P< 0.05), phosphatidylinositol(PI) and sphingomyelin(SM) showed no significant difference(P>0.05). Conclusion The level of red cell membrane phospholipids participation influence the development process of acute ischemic stroke, and may be an inducing mechanism of the development of acute ischemic stroke.更多还原