【摘要】 目的探讨白术对D-半乳糖致脑老化小鼠学习记忆及海马突触结构的影响。方法采用连续皮下注射D-半乳糖的方法,建立脑老化小鼠模型,通过Morris水迷宫检测小鼠的记忆能力,用透射电镜和形态计量法分析白术对脑老化小鼠CA3区Gray I型突触结构参数的影响。结果水迷宫实验:脑老化组的逃避潜伏期显著长于对照组P<0.05,而RAM低、中、高组的逃避潜伏期显著短于脑老化组(P<0.05,P<0.01。海马CA3区Gray I型突触结构观察:与对照组相比,脑老化组小鼠的突触界面曲率、突触后致密物厚度均显著减小(P<0.05;P<0.01),突触间隙宽度显著增大(P<0.05);白术组小鼠的突触界面曲率、突触间隙宽度、突触后致密物厚度与对照组基本相近似(P>0.05)。结论白术可以改善脑老化所致小鼠海马CA3区Gray I型突触结构的改变,改善学习记忆能力。更多还原

【Abstract】 Objective To observe the effects of rhizoma atractylodis macrocephalae(RAM) on learning-memory and synaptic structure in D-galactose-induced brain aging mice. Methods D-galactose was injected subcutaneously for the brain aging model. Learning-memory ability was tested by Morris water maze test and The structure of Grey I type synapses in hippocampal CA3 area were observed under transmitted electron microscope. Results Morris water maze test: In brain aging group, the escape latency was significantly prolonged,(P<0.05). The escape latency in 3 RAM group was significantly shorter(P<0.05,P<0.01). The structure of Grey I type synapses in hippocampal CA3 area. In brain aging group, the curvature of synapse interface and thickness of postsynaptic desity were significantly decreased(P<0.05, P<0.01), and the width of synaptic cleft was significantly increased(P<0.05) compared with control group. In RAM-treated groups, the curvature of synapse interface, width of synaptic cleft and thickness of postsynaptic desity were approximately the same as that of control group(P>0.05). Conclusion RAM could significantly increase the ability of learning-memory in brain aging mice. the mechanism related to the protective effect on synapese in hippocampal CA3 area.更多还原