【摘要】 目的观察β连环蛋白(β-catenin)、血管内皮生长因子(VEGF)及金属基质蛋白酶9(MMP-9)在子宫内膜异位症(EMs)中的表达,为完善其病理机制,寻找新靶点提供依据。方法以39例EMs患者为病理对象,根据r AFS分期分为轻度EMs(Ⅰ、Ⅱ期,17例)和重度EMs(Ⅲ、Ⅳ期,22例)两组,并以30例无内膜病变的卵巢囊肿患者为对照;所有患者均行腹腔镜手术,术中留取子宫内膜组织,采用免疫组化实验观察β-catenin、VEGF及MMP-9的表达率和表达强度。结果重度EMs、轻度EMs、对照组的β-catenin阳性表达率分别为86.4%、64.7%、53.3%,VEGF阳性表达率均为100%,MMP-9阳性表达率分别为81.8%、76.5%、50.0%,其中重度EMs的β-catenin和MMP-9阳性表达率均显著高于对照组(P<0.05);重度EMs的β-catenin、VEGF及MMP-9的阳性表达强度高于轻度EMs,轻度EMs的β-catenin、VEGF及MMP-9的阳性表达强度高于对照组,差异均有统计学意义(P<0.05)。结论β-catenin、VEGF及MMP-9在子宫内膜异位症的发病机制中介导了重要环节,有望成为其临床治疗的新靶点。更多还原

【Abstract】 Objective To understand the expression levels ofβ-catenin,VEGF and MMP-9 in endometriosis( EMs) so as to provide data for completing its pathomechanism and searching for new target. Method 39 EMs patients were divided into 2groups according to r AFs stages: 17 patients in stageⅠandⅡwere named mild Ems group,and 22 patients in stage Ⅲ and Ⅳwere named severe Ems group. At the same time,30 ovarian cyst patients without endometrial lesions were selected as the control group. All the patients were treated with laparoscopic surgery,where endometrial tissues were extracted in the surgery and detected forβ-catenin,VEGF and MMP-9 expression using immunohistochemical experiment. Findings The β-catenin positive expression rate of the severe Ems group,the mild Ems group and the control group were 86. 4%,64. 7% and 53. 3%respectively. The VEGF positive expression rate of all the groups was 100%. The MMP-9 positive expression rate of the severe Ems group,the mild Ems group and the control group were 81. 8%,76. 5% and 50. 0%. Theβ-catenin and MMP-9positive expression rate of the severe Ems group were both higher than those of the mild Ems group,and those of the mild Ems group were both higher than those of the control group. The difference between groups had statistical significance( P <0. 05). Conclusion β-catenin,VEGF and MMP-9 take important part in the pathogenesis of endometriosis,which are expected to become the new targets for its clinical treatment.更多还原