【摘要】 针对我国目前缺乏荧光猝灭法光学溶解氧传感器校准方法的现状,以AADI 4330F溶解氧传感器为实验对象,提出了一种适用于所有荧光猝灭法光学溶解氧传感器的实验室校准方法。该方法使用一个能够精确控制水体温度的自制校准装置,通过改变通入容器的氧气和氮气比例来控制容器内的溶解氧含量,在4个温度下分别测定至少10组水体温度值、传感器相位值和Winkler碘量法测定值数据,使用回归分析进行多项式拟合获取传感器校准系数。经过实验室验证,在校准试验温度范围内随机温度和溶解氧浓度下,使用该方法校准后传感器测定值与标准值偏差在±5μmol/L以内,达到了仪器自身的准确度水平。本方法在校准结果准确度和温度适用范围等方面都明显优于两点校正法,表明该方法具有良好的校准效果和推广应用价值。更多还原

【Abstract】 Recently, oxygen sensors have been widely used in marine research, especially in studying the mass transfer rate of O₂ across the sediment-water interface, net community production of deep ocean and hyperbaric trap-respirometer for the capture and maintenance of deep-sea organisms. Most currently deployed oxygen sensors drift for months on the surface ocean and must be calibrated to maintain their accuracy. Focused on the fact that calibration methods for dissolved oxygen sensors based on fluorescence quenching are absent in China,this paper, with the the AADI 4330 F dissolved oxygen sensor as the object of study, presents an oxygen calibration system designed to work in the laboratory using Aanderaa optode oxygen sensors. This method uses a self-made calibration apparatus that can accurately control the water temperature and regulate the dissolved oxygen content in the container through adjusting the O₂/N₂ ratio. At least 10 groups of water temperature values,sensor phase values and Winkler titration values are measured under 4 temperatures, and the sensor calibrating coefficient is obtained through polynomial fitting with regression analysis. Laboratory validation proves that, under the random temperature and dissolved oxygen level within the calibrating temperature scope, the deviation between the measured values and standard values is well within ±5 μmol/L with the use of this method, achieving the precision requirement. The presented method obviously precedes the two-point calibrating method in the aspects of result accuracy and temperature range, and thus has good calibrating effects and application value.更多还原