【摘要】 本文采用气相质谱联用技术对三株海水微藻—三角褐指藻(Phaeodactylum tricornutum,CCMM2012)、小球藻(Chlorella vulgaris,CCMM4004)、微拟球藻(Nannochloropsis sp.,CCMM6004),和一株淡水微藻—栅藻(Scenedesmus quadricauda,CCMM4002)在不同温度下的生长及油脂变化规律进行研究。结果表明:三角褐指藻、小球藻和栅藻在20℃下的油脂产率最大,分别为2.94、5.79和5.83mg/L/d,它们在20℃的培养条件下比较适合应用于生物能源开发;而微拟球藻在25℃下油脂产率最大,达6.45mg/L/d,因此此温度下更适合用于生物能源开发。三角褐指藻在15℃条件下DHA和EPA含量最多,为16.47%,而微拟球藻在25℃下DHA和EPA最多,为22.74%;因此三角褐指藻和微拟球藻在15℃和25℃下均比较适合应用于多不饱和脂肪酸的开发,而小球藻和栅藻的EPA和DHA含量在三个温度下普遍较少,不适合用于多不饱和脂肪酸的开发。更多还原

【Abstract】 Growth and lipid change in three marine microalgae(Phaeodactylum tricornutum, Chlorella vulgaris, and Nannochloropsis sp.) and a freshwater microalga(Scenedesmus quadricauda) under different temperatures(15, 20, and 25℃) were studied by GC-MS. The results show that the lipid production in P. tricornutum, C. vulgaris and S. quadricauda peaked at 2.94, 5.79, and 5.83mg/L/d, respectively, and these three algae shall be suitable for bioenergy preparation. For Nannochloropsis sp., the peak point of 6.45mg/L/d occurred in 25 ℃. The contents of EPA and DHA in P. tricornutum were the highest at 16.47% in 15 ℃, while for Nannochloropsis sp., they reached maximum at 22.74% in 25 ℃. Therefore, P. tricornutum and Nannochloropsis sp. were potential appropriate microalgae for production of polyunsaturated fatty acid. However, the contents of EPA and DHA of C. vulgaris and S. quadricauda were very low under the three temperatures. Therefore, using them for polyunsaturated fatty acid would be inappropriate.更多还原