【摘要】 目的建立熊胆川贝枇杷膏的质量标准。方法采用薄层色谱法鉴别熊胆粉、枇杷叶和薄荷脑药材,并用HPLC法测定制剂中的牛磺熊去氧胆酸及岩白菜素的含量。结果薄层色谱特征斑点清晰,阴性样品无干扰。0.05~0.73 mg·m L-1牛磺熊去氧胆酸、7.7~154μg·m L-1岩白菜素与峰面积呈良好的线性关系(r=0.9999),平均回收率分别为98.0%、99.2%,RSD分别为0.75%、0.65%。结论所用方法简便、准确、专属性好,可作为熊胆川贝枇杷膏的质量控制方法。更多还原

【Abstract】 OBJECTIVE To establish the quality standard for Xiongdan Chuanbei Pipa cream( XCPC). METHODS The TLC method was used to identify bear gall powder,loquat leaf and menthol. The contents of sodiumtauroursodeoxycholate and bergenin were determined by HPLC. RESULTS The TLC spots were clear and the negative test showed no interference. For sodium tauroursodeoxycholate,the calibration curve was linear within the concentration range of 0. 05-0. 73 mg·m L-1( r = 0. 9999). The average recovery was 98. 0%,and the RSD was 0. 75%. For bergenin,the calibration curve was linear within the concentration range of 7. 7-154 μg·m L-1( r = 0. 9999). The average recovery was 99. 2%,and the RSD was 0. 65%. CONCLUSION These methods are simple,accurate,specific,and can be used for the quality control of XCPC.更多还原