【摘要】 目的观察灯盏花乙素(scutellarin)对肝星状细胞(hepatic stellate cell,HSC)及转化生长因子β1(TGF-β1)/Smad信号通路的影响,探讨scutellarin抗肝纤维化的机制。方法建立TGF-β1诱导的HSC LX-2模型,CCK-8法检测scutellarin对细胞增殖的作用,化学发光法检测scutellarin对Caspase3/7活性的影响,酶联免疫吸附测定(enzyme linked immunosorbent assay,ELISA)法检测细胞上清液Ⅰ型前胶原的含量,ELISA法检测细胞Smad2和Smad3磷酸化水平。结果scutellarin能抑制HSC增殖,增强细胞Caspase3/7活力,抑制Ⅰ型前胶原分泌,降低LX-2细胞Smad2和Smad3磷酸化水平。结论抑制TGF-β1/Smad信号通路可能是scutellarin抗肝纤维化的作用机制。更多还原

【Abstract】 Objective To observe the effect of scutellarin on signaling pathway in hepatic stellate cells(HSC)and transforming growth factor beta 1(TGF-beta 1),to explore the mechanisms of antihepatic fibrosis.Methods Experiments were conducted by human HSC cells line.Transforming growth factor-β1(TGF-β1)was used to induce HSC model.The inhibitory effect of scutellarin on LX-2 cells was observed.Chemiluminescence measurement was used to measure the activity of caspase3/7 in LX-2 cells.ELISA was used to detect the content of pro-collagen Ⅰ in LX-2 supernatant.The expressions of PhosphoSmad2 and Phospho-Smad3 were measured by ELISA.Results Scutellarin could Inhibit LX-2 cells proliferation,pro-collagen,I secretion,and phosphorylation of Smad2,Smad3 expressions.Scutellarin could improve the activities of caspase3/7.Conclusion The mechanism of scutellarin to anti hepatic fibrosis may be the inhibition of TGF- beta 1/Smad signaling pathway更多还原