【摘要】 对9种代表性Cry1A型杀虫晶体蛋白成员进行活性区空间结构的同源模建与比较分析.分析结果表明:DomainⅠ和DomainⅢ相对保守,其中,DomainⅠ整体走向及结构基本重叠,只有Cry1Aa和Cry1Af多了一个螺旋;DomainⅡ的主要差异体现在loop上;将DomainⅢ结构一致的成员Cry1Ab,Cry1Ad,Cry1Ae和Cry1Af归为一个亚型,其他5种成员归为另一个亚型.研究确定了影响Cry1A型杀虫晶体蛋白结构差异的关键氨基酸及关键结构片段.更多还原

【Abstract】 The 3-D structure of the toxic core for all available 9typical Cry1 Aproteins were constructed by the method of homology modeling.The structural differences among different Cry1 Aproteins indicated that Domain Ⅰ and Domain Ⅲ were more conservative than Domain Ⅱ.Structures of Cry1 A Domain Ⅰ were almost identical except Cry1 Aa and Cry1 Af which had one more helix.The differences among the Cry1 ADomain II were mainly located in loops.Structures of DomainⅢ of Cry1 Ab,Cry1Ad,Cry1 Ae and Cry1 Af were consistent,thus these four Cry1 Aproteins and the other five were divided into two different subgroups.The results confirm that the key residues and motifs are important for the insecticidal activites of Cry1 Aproteins.更多还原