【摘要】 目的:研究TNFRSF1B+676位点(rs1061622)基因多态性对湖南汉族人群类风湿关节炎(rheumatoid arthritis,RA)风险的影响。方法:标本来源于112例湖南汉族RA患者及129例健康献血者。TNFRSF1B+676基因多态性采用限制性片段长度多态性(PCR-RFLP)法分析。血清可溶性肿瘤坏死因子受体II(soluble tumor necrosis factor receptor II,sT NFR II)水平检测采用ELISA方法。结果:湖南汉族人群中,RA组TNFRSF1B+676基因型构成与正常对照组比较无明显差异(GG/TG/TT:5/62/45 vs 9/56/64,P=0.167);女性RA患者TNFRSF1B+676基因型构成比与女性正常对照比较差异有统计学意义(GG/TG/TT:3/49/24 vs 8/28/48,P<0.001);RA组TNFRSF1B+676基因位点T或G等位基因频率与正常对照组比较无明显差异(P>0.05)。RA患者血清sT NFR II水平明显高于正常对照组[(7.83±2.61)ng/mL vs(4.32±1.67)ng/mL,P<0.001);无论是RA患者或正常对照,TNFRSF1B+676位点GG,TG,TT三种不同基因型血清s TNFR II水平比较,差异无统计学意义(P>0.05)。结论:在湖南汉族人群中,TNFRSF1B+676基因多态性与RA易感性无明显关联。更多还原

【Abstract】 Objective: To study the association of TNFRSF1 B +676 gene(rs1061622) polymorphism with the risk of rheumatoid arthritis(RA) in Han Chinese population of Hunan.Methods: A total of 112 patients with RA from Han Chinese population in Hunan were recruited, along with 129 healthy controls. TNFRSF1 B +676(rs1061622) gene polymorphisms were examined by PCR-RFLP. Serum levels of soluble TNFR II were analyzed by ELISA.Results: RA patients displayed a similar TNFRSF1 B +676 genotype to controls(GG/TG/TT: 5/62/45 vs 9/56/64, P=0.167), but significant difference was found between female RA patients and female controls(GG/TG/TT: 3/49/24 vs 8/28/48, P<0.001). No significant difference was found in the frequency of TNFRSF1 B +676 T or G allele between RA patients and controls(P>0.05). RA patients showed a significantly higher level of serum soluble tumor necrosis factor receptor II(sT NFR II) than controls [(7.83±2.61) ng/mL vs(4.32±1.67) ng/mL, P<0.001], but there was no difference among the three genotypes(P>0.05). No association was found between TNFRSF1B+676 gene polymorphism and RA clinical characteristics.Conclusion: In Han Chinese population of Hunan province, TNFRSF1B+676 gene polymorphisms are not associated with the genetic risk of RA.更多还原