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氯化镧对铜胁迫下玉米幼苗抗氧化特性的影响

Effects of Lanthanum Chloride on Antioxidant Characteristics of Maize Seedlings under Copper Stress

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【作者】 代惠萍赵桦贾根良吴三桥赵美容

【Author】 DAI Huiping;ZHAO Hua;JIA Genliang;WU Sanqiao;ZHAO Meirong;Shaanxi Province Key Laboratory of Bio-resources,Shaanxi University of Technology;College of Biological Science & Engineering,Shaanxi University of Technology;School of Life Sciences,Lanzhou University , Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution;College of Science,Northwest A&F University;

【机构】 陕西理工学院/陕西省资源重点实验室陕西理工学院生物科学与工程学院兰州大学生命科学学院/甘肃省环境生物监测与修复重点实验室西北农林科技大学理学院

【摘要】 以玉米为试材,采用溶液培养方法,研究了外源氯化镧对铜胁迫下玉米幼苗叶片及根系抗氧化特性的影响。结果表明,铜胁迫显著提高了玉米叶片SOD、CAT、APX、DHAR活性、GSH含量、细胞质膜透性、MDA含量及根系SOD、CAT、DHAR活性、As A含量、细胞质膜透性、MDA含量,而使叶片POD、GR活性、As A含量及根系POD、APX、GR活性、GSH含量显著降低。与铜胁迫相比,氯化镧+铜复合处理叶片的SOD、APX、GR、DHAR活性与As A含量分别显著增加了16.3%、86.2%、225.0%、62.5%、267.7%,其根系POD、APX、GR、DHAR活性和GSH含量分别显著增加了148.5%、66.7%、51.5%、23.7%、19.3%,叶片CAT、POD活性、GSH含量、细胞质膜透性、MDA含量分别降低了47.5%、15.0%、15.4%、44.3%、48.1%,根系CAT活性、As A含量、细胞质膜透性、MDA含量分别降低了42.9%、50.0%、35.8%、41.1%,氯化镧+铜复合处理使玉米幼苗生物量显著增加了25.4%。综上,氯化镧可提高玉米幼苗的抗氧化能力,从而缓解铜胁迫玉米植株所造成的伤害。

【Abstract】 Hydroponic experiments were conducted to study the effects of exogenous lanthanum chloride on the antioxidant properties of maize seedling leaves and roots under copper stress. The results showed that copper stress significantly increased the activities of SOD,CAT,APX,DHAR,membrane permeability,and the contents of GSH,MDA in leaves,as well as the activities of SOD,CAT,DHAR,membrane permeability,and contents of As A,MDA in roots,however,significantly decreased the activities of POD,GR and content of As A in leaves,as well as the activities of POD,APX,GR and the GSH content in root. Compared with copper stress alone,exogenous lanthanum chloride could significantly increase the activities of SOD,APX,GR,DHAR and As A content by 16. 3%,86. 2%,225. 0%,62. 5%,267. 7% respectively in leaves,andthe activities of SOD,POD,APX,GR,DHAR and the GSH content by 1. 6%,148. 5%,66. 7%,51. 5%,23. 7%,19. 3% respectively in roots; decrease the activities of CAT,POD,membrane permeability,and content of GSH,MDA by 47. 5% 、15. 0% 、15. 4% 、44. 3% 、48. 1% in leaves,the activities of CAT,membrane permeability,and content of As A,MDA in roots by 42. 9%,50%,35. 8%,41. 1%; and significantly increase the biomass per plant by 25. 4%. Above results indicated that lanthanum chloride could alleviate the oxidative damage and enhance the antioxidant abilities of maize seedlings.

【基金】 陕西省教育厅重点实验室科研计划项目(15JS019);陕西省自然基金项目(2015JM3086);甘肃省环境生物监测与修复重点实验室开放基金项目(GBBL2015006);国家大学生创新创业训练计划项目(201510720570);陕西省大学生创新创业训练计划项目(1970);陕西理工学院大学生创新创业训练计划项目(UIRP15034)
  • 【文献出处】 河南农业科学 ,Journal of Henan Agricultural Sciences , 编辑部邮箱 ,2016年04期
  • 【分类号】S513
  • 【被引频次】3
  • 【下载频次】152
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