【摘要】 以溴化乙锭(EB)做为DNA的荧光探针,研究了黄酮类化合物——槲皮苷与DNA的相互作用。实验结果表明,离子强度对槲皮苷与DNA的结合常数有一定的影响。在15℃、25℃、35℃和45℃时的Stern-Volmer曲线说明了槲皮苷对DNA荧光猝灭的过程。通过计算得到了在不同温度时二者的结合常数,以及相对应的热力学常数ΔH、ΔG和ΔS。更多还原

【Abstract】 Binding of flavonoids,using quercitrin to fish sperm deoxyribonucleic acid( DNA) was studied by using Ethidium Bromide( EB) as a fluorescence probe. The experimental results indicated that the ionic strength had effect on the binding of quercitrin and DNA. Stern-Volmer plots at 15 ℃,25 ℃,35 ℃ and 45 ℃ showed that the quenching of fluorescence by quercitrin was a combined quenching process. The apparent binding constant KAat different temperatures and the corresponding thermodynamic parameters ΔH,ΔG and ΔS were obtained.更多还原