To clone the vascular endothelial growth factor D( VEGF- D) genes from small- tailed Han sheep and Xinji fine- wool sheep,and detect the polymorphism( SNP) and distribution law of tissue expression pattern,and explore whether there was a necessary connection between the VEGF- D genes and difference in the wool traits of the two varietis of sheep,total RNAs were extracted from skin and different tissues in small- tailed Han sheep and Xinji fine- wool sheep. The VEGF- D genes were cloned by RT- PCR and used f...
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