To study the immunogenicity of VP1 protein of Foot- and- mouth disease virus( FMDV) type A,a target gene VP1 was amplified from the plasmid p MD- 18- P1 of foot- and- mouth disease( FMD) using PCR assay. The target gene VP1 was linked with prokaryotic expression vector p ET- 28 a vector to construct a recombined plasmid p ET- 28a- VP1,and then the recombined plasmid was transformed into Escherichia coli Rosetta( DE3) competent cells. The positive colonies were screened,and then the expression of protein was...
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