To study the role of the modification status of protein phosphosrylation status in the process of somatic cells induced to pluripotent stem cells,a technique for Ti O2 enrichment of phosphorylated peptides and high- throughput mass spectrometry technique were used for qualitative detection and bioinformatics analysis of the phosphoproteome from the third- passage bovine fetal fibroblasts( BFFs). The results showed that a total of 363 phosphoproteins with 502 phosphorylation sites were identified in the samp...
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