【摘要】 探讨了生殖老化对小鼠卵母细胞后期发育及基因表达水平的影响。结果表明,与青年小鼠相比,老龄小鼠MII期卵母细胞经体外受精后早期胚胎原核形成的时间发生延迟(1 h),囊胚发育率有显著差异(61.98%vs.70.95%,P<0.05)。提取老龄和青年CD1小鼠MII期卵母细胞总RNA,使用基因表达谱芯片对其进行分析。结果表明,生殖老化导致1 737个基因表达表现出显著差异,其中64.9%的基因表达上调,35.1%的基因表达下调,生殖老化导致Hdac10等基因产生极显著的表达差异,小鼠卵母细胞质量下降,最终影响其后期发育能力。更多还原

【Abstract】 The purpose is to investigate the correlation between the old and young mouse oocyte early embryonic development and the molecular alteration of the old mouse oocyte. Compared with young mice oocytes,the early embryonic pronucleus formation of old mice oocytes after in vitro fertilization( IVF)delays 1 h,ratios of blastocysts appear significant difference( IVF: 61. 98% vs. 70. 95%,P < 0. 05).Furthermore,results of cRNA microarray- based analysis show that within the increasing of age,1737 genes expression showed significant difference( fold- change is not less than 2). Ratios of up- regulated and down- regulated genes are 64. 9% and 35. 1% respectively,which make oocyte quality decline.Therefore,differentially expression of genes which affected by the reproductive aging,such as Hdac10,makes oocyte quality decline,and has an great impact on the development competence ultimately.更多还原