【摘要】 目的研究过氧化物酶体增殖物受体γ共激活因子1α(PGC-1α)在铅诱导的小鼠睾丸支持细胞能量代谢障碍中的作用。方法将小鼠睾丸支持细胞(TM4)及其过表达细胞(PGC-1α(+)TM4)、低表达细胞(PGC-1α(-)TM4)分别暴露于含乙酸铅终浓度为0(对照组)、10、20、40、80、160μmol/L的培养基培养24 h。分别测定细胞内三磷酸腺苷(ATP)含量、乳酸(LD)的含量和乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)的活力。结果与对照组相比,各浓度TM4及其过表达、低表达细胞内细胞内ATP、LD的含量和LDH、SDH的活力均下降,差异有统计学意义(P<0.05);且随着乙酸铅染毒浓度升高,TM4及其过表达、低表达细胞内ATP、LD的含量和LDH、SDH的活力均呈下降趋势。当乙酸铅染毒浓度相同时,3种细胞内ATP、LD的含量和LDH、SDH的活力依次为PGC-1α(+)TM4>TM4>PGC-1α(-)TM4,差异均有统计学意义(P<0.05)。结论 PGC-1α对铅诱导的小鼠睾丸支持细胞能量代谢障碍具有一定的拮抗作用。更多还原

【Abstract】 Objective To explore the role of PGC-1α(peroxisome proliferator-activated receptor-γ-coactivator-1α) in protection against lead-induced energy metabolism dysfunction in TM4 cells. Methods The mouse Sertoli cells(TM4),PGC-1α overexpression cells [PGC-1α(+)TM4] and PGC-1α knockdown cells [PGC-1α(-)TM4] were treated with lead acetate at the doses of 0(control group),10,20,40,80 and 160 μmol/L respectively for 24 hours. Then adenosine triphosphate(ATP) content,lactic acid(LD) content,lactic dehydrogenase(LDH) activity and succinic dehydrogenase(SDH) activity were determined.Results Compared with the control group,ATP content,LD content,LDH activity and SDH activity in TM4 cells,PGC-1α(-)TM4 cells and PGC-1α(+)TM4 cells significantly decreased(P<0.05). And with the increase of dose of lead acetate,in TM4 cells,PGC-1α(-)TM4 cells and PGC-1α(+)TM4 cells,ATP content,LD content,LDH activity and SDH activity showed a decreasing trend. When treated with the same concentration of lead acetate,ATP content,LD content,LDH activity and SDH activity in the three cell lines were PGC-1α(+)TM4> TM4 > PGC-1α(-)TM4(P<0.05). Conclusion PGC-1α may play a protective role in lead-induced energy metabolism dysfunction in TM4 cells.更多还原