【摘要】 以海水为基质,采用传统的微生物分离纯化方法,从海底沉积物中分离筛选得到1株耐盐异养硝化-好氧反硝化细菌y5,经形态、生理生化特性以及16S rRNA基因序列分析,鉴定该菌为克雷伯氏菌(Klebsiella sp.).对其脱氮特性及影响因素进行了研究,结果表明,菌株y5的最佳碳源为柠檬酸三钠,最适p H值为7.0,最适C/N为17.菌株均能以NH4Cl、Na NO2和KNO3为唯一氮源进行反应,36 h的去除率分别为77.07%、64.14%和100%.3种氮源共存时,36 h的去除率达到100%.表明菌株y5在高盐废水中具有独立高效的异养硝化和好氧反硝化作用.更多还原

【Abstract】 A heterotrophic nitrification-aerobic denitrification strain named y5 was isolated from marine environment by traditional microbial isolation method using seawater as medium. It was identified as Klebsiella sp. based on the morphological,physiological and16 S rRNA sequence analysis. The experiment results showed that the optimal carbon resource was sodium citrate; the optimal p H was7. 0; and the optimal C / N was 17. The strain could use NH4 Cl,Na NO2 and KNO3as sole nitrogen source,and the removal efficiencies were77. 07%,64. 14% and 100% after 36 hours,respectively. The removal efficiency reached 100% after 36 hours in the coexistence of NH4 Cl,Na NO2 and KNO3. The results showed that the strain y5 had independent and efficient heterotrophic nitrification and aerobic denitrification activities in high salt wastewater.更多还原