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除铊硫酸盐还原菌固定化的优化和表征
OPTIMIZATION AND CHARACTERIZATION OF IMMOBILIZED SULFATE REDUCING BACTERIA FOR THALLIUM REMOVAL
【摘要】 采用海藻酸钠和聚乙烯醇等为包埋剂,用包埋法对SRB细菌进行固定化,以固定化小球处理含铊废水的能力和失重率为主要参考指标,通过正交试验确定固定化硫酸盐还原菌(SRB)的最佳比例,并通过研究溶胀度和处理能力来评估该材料的性能。研究发现固定化SRB的最佳包埋比例为:聚乙烯醇为6%,二氧化硅为3%,海藻酸钠为0.5%,活性炭为2%,菌液含量为35%,饱和硼酸中氯化钙为2%。固定化SRB对KCl和Fe Cl3溶液有较强的敏感度,和未包埋细菌空白小球相比,固定化SRB对含铊废水有较强的处理能力,最大处理量能达到207.35 mg/g。通过电镜扫描发现,固定化SRB表面和内部布满密集的微孔,对含铊废水处理起到关键的作用。
【Abstract】 Polyvinyl alcohol and sodium alginate were selected as entrapping agents to immobilize sulfate reducing bacteria( SRB). The abilities of adsorbing thallium and weightlessness rate of immobilized SRB were taken as the mainly parameters to determine the optimal conditions of immobilized SRB by means of orthogonal experiment. The swelling degree and the adsorption capacity were studied to evaluate the material performance. According to the experiment,the optimal conditions of imbedding immobilized SRB were: Polyvinyl alcoholof 6%,sodium alginate of 0. 5%,silicon dioxide of 3%,activated charcoal of 2%,bacteria of 35%,Ca Cl2 of 2%. Immobilized SRB had a stronger sensitively to KCl and Fe Cl3 solution. The adsorption capacity of immobilized SRB could reach 207. 35 mg/g during the course of treating wastewater containing thallium,which was far greater than blank bead. A large number of pores in the surface and inner of immobilized SRB could be found by scanning electron microscope,which played a key role in treating wastewater containing thallium.
【Key words】 immobilization; sulfate reducing bacteria; thallium; wastewater;
- 【文献出处】 环境工程 ,Environmental Engineering , 编辑部邮箱 ,2016年09期
- 【分类号】X703;X172
- 【被引频次】1
- 【下载频次】207