Acetohydroxy acid synthase(AHAS) and acetohydroxy acid isomeroreductase(AHAIR) encoded by ilv BN and ilv C are two key enzymes which play important roles in the biosynthetic pathway of L-valine.Brevibacterium flavum MD515 was used as the origin strain and site-specific mutagenesis was performed in its ilv N gene which coded for the regulatory subunit of AHAS,resulting in the obtainment of an anti-feedback inhibition gene,named ilv BN'C.Then,the ilv BNrC gene was ligased to plasmid p Z8-1 for construction of...
【更新日期】
2016-07-21
【分类号】
TQ922
【正文快照】
Received date:2015-12-21.Corresponding author:WANG Mingzi,mingziw@fjnu.edu.cn引言L-缬氨酸(valine)是一种支链氨基酸,广泛应用于医药、食品及调味剂、动物饲料和化妆品等行业[1-3]。目前,发酵法、直接提取法和化学合成法是工业生产L-缬氨酸的3种主要方法[4]。微生物发酵?
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