【摘要】 木犀草素是一种大量存在于蔬菜、水果以及天然药草中的天然黄酮类化合物,具有抗炎、抗氧化、改善胰岛素抵抗和免疫调节等作用。肥胖常伴随非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)的发生,因此研究木犀草素改善NAFLD具有极其重要的意义。文章用高脂饮食(high fat diet,HFD)喂养C57BL/6小鼠20周来建立非酒精性脂肪肝小鼠模型,同时设立低脂饮食(low fat diet,LFD)作为正常对照组,高脂添加0.01%木犀草素(HFD+Lut)作为实验组。用苏木精伊红染色和油红染色检测肝脏中脂质积累,用实时定量荧光聚合链式反应(real-time polymerase chain reaction,Real-Time PCR)检测脂质合成基因及其转录因子相关基因的表达。结果表明,木犀草素能够减少小鼠肝脏中脂质积累,并且能抑制小鼠肝脏内脂质合成的基因以及转录因子的表达,探明了木犀草素能改善HFD导致的NAFLD及其作用机制。更多还原

【Abstract】 Luteolin is a natural flavonoid compound which exists in fruits,vegetables and natural herbs.It has been reported that luteolin has the properties of anti-inflammation,anti-oxidation,improving insulin sensitivity and immune regulation.Obesity is often accompanied with non-alcoholic fatty liver disease(NAFLD),so it is of importance to study the effect of luteolin on NAFLD.In this paper,the NAFLD model of C57BL/6mice induced by high fat diet(HFD)for 20 weeks was established.The mice fed with low fat diet(LFD)were set as normal control group,and the mice fed with HFD adding 0.01%luteolin were set as the test group.The liver lipid accumulation was detected by using the hematoxylin and eosin(HE)staining and Oil Red O staining procedures.And the lipid metabolism-related gene expression was quantified by using real-time polymerase chain reaction(PCR).The results showed that luteolin decreased the lipid accumulation in liver of mice,and improved the expression of genes related to lipid synthesis and transcription factors.The presented study verified the effect of luteolin on the improvement of NAFLD caused by HFD and the possible mechanism of action.更多还原