【摘要】 目的探讨人参皂苷Rg1对大鼠离体心脏缺血再灌注损伤的减轻作用及机制。方法制备大鼠离体心脏I/R模型,将18只大鼠随机分为正常对照组、I/R组、人参皂苷Rg1组。100μmol/L人参皂苷Rg1进行预处理10 min,观察血流动力学指标左心室内压最大上升/下降速率和心率压力乘积的变化,检测灌流液中肌酸激酶(CK)、乳酸脱氢酶(LDH)活性。Western blotting检测心肌组织PI3K下游激酶Akt、ERK1/2及其磷酸化水平的变化,磷酸化GSK-3β的蛋白表达。结果与I/R组比较,人参皂苷Rg1预处理显著改善心脏功能,降低灌流液中CK、LDH活性(P<0.05);升高p-Akt和p-GSK-3β的蛋白表达(P<0.05)。结论人参皂苷Rg1对大鼠离体心脏缺血再灌注损伤有减轻作用,其机制与激活PI3K-Akt通路有关。更多还原

【Abstract】 Objective To investigate cardioprotection effects of Ginsenoside Rg1 on ischemia/reperfusion( I/R)injury in isolated hearts of rats,and to explore its action mechanism. Methods The Langendorff models of myocardial I / R injury in rats were established. The 18 rats were randomly divided into three groups: control group,I / R group and Ginsenoside Rg1 group,with 6 rats in each group. The rats ’hearts in Ginsenoside Rg1 group were pretreated with Ginsenoside Rg1100μmol / L for 10 min. The cardiac indexes including maximal rise / fall of left ventricular pressure( ± dp / dtmax) and rate pressure product( RPP) were measured,and the activities of creatine kinase( CK) and lactate dehydrogenase( LDH) were detected. The changes of phosphorylation of protein kinase B / Akt,and its downstream target glycogen synthase kinase 3β( GSK-3β) and extracellular signal-regulated kinase1 /2( ERK1 /2) were detected after 10- minute reperfusion. Results As compared with those in I / R group,the activities of CK,LDH in Ginsenoside Rg1 group were significantly decreased and cardiac function was obviously improved( P < 0. 05),moreover,the expression levels of p-Akt and p-GSK-3βwere significantly increased( P < 0. 05). Conclusion Ginsenoside Rg1 can alleviate I / R injury in isolated hearts of rats,and its action mechanism may be correlated to the activation of PI3K-Akt signaling pathway.更多还原