【摘要】 利用表型性状和ISSR分子标记技术对3份白花苜蓿、3份黄花苜蓿、5份紫花苜蓿和3份扁蓿豆共计14份苜蓿属材料进行研究,分析其遗传关系,进一步解析其之间的分类学关系。从表型性状来看,白花苜蓿区别于其他苜蓿的主要形态学特征是花色为乳白色,荚果1.0~1.5个螺旋,从聚类分析来看,3份扁蓿豆材料和11份苜蓿材料非常明显的聚成了2类,说明扁蓿豆与苜蓿还是有较大的区别,在遗传距离15的地方,白花苜蓿、紫花苜蓿和黄花苜蓿各单独聚为1类;从亲缘关系来看,扁蓿豆与黄花苜蓿的遗传距离最近,与紫花苜蓿的最远,白花苜蓿次之,相对于紫花苜蓿,白花苜蓿与黄花苜蓿的遗传距离较近;从ISSR分子标记水平来看,4类苜蓿种质资源在遗传距离0.80的地方分别聚为单独的4类,从遗传关系来看,白花苜蓿相对于黄花苜蓿与紫花苜蓿的遗传关系更近,但紫花苜蓿与白花苜蓿和黄花苜蓿的遗传关系几乎相等。更多还原

【Abstract】 Morphology and ISSR Marker and their clusters analysis were carried out in 5 M. sativa L.,3 M.falcata L.,3 alfalfa with cream flower and 3 M. ruthenica L. for analysing the genetic relationship to further reveal the taxonomic relationship. The results showed alfalfa with cream flower had the significant differences in flower color( cream) and pod( 1. 0-1. 5 coils). In cluster analysis,2 groups were clustered between 3 M. ruthenica L. and11 Medicago L. which meant the significant at the genetic distance of 15; at the genetic distance of 10,3 groups were clustered in 5 M. sativa L.,3 M. falcate L. and 3 alfalfa with cream flower. From the relationship,M. ruthenica L. had the closer relationship than that of M. sativa L. and alfalfa with cream flower; alfalfa with cream flower had the closer relationship than that of M. sativa L.. At the genetic distance of 0. 80,4 groups were clustered in 5 M. sativa L.,3 M. falcata L.,3 alfalfa with cream flower and M. ruthenica L. in ISSR Marker,by the genetic relationship,alfalfa with cream flower had the closer genetic relationship with M. sativa L. comparing with M. falcate L.,but,M.sativa L. had the equal genetic relationship with alfalfa with cream flower and with M. falcate L..更多还原