【摘要】 作为木本植物分子生物学和基因工程的模式树种,杨树基因工程研究进展较快。文章对杨树抗虫基因工程20多a来在多方面取得的研究进展,包括转不同类型抗虫基因抗虫效果、毒蛋白表达研究、多基因聚合、转抗虫基因杨树抗虫稳定性及生物安全性等方面进行了综合评述;提出了多基因转化中仍存在个别基因丢失或基因沉默等问题,并对多个外源基因在植物中如何同时高效表达的策略,尤其是构建载体时应改善的方法进行了探讨。更多还原

【Abstract】 Poplar is a pattern tree species of woody plant molecular biology and genetic engineering,and the study of gene engineering for poplar developed rapidly.This paper provided a comprehensive review of the research progress in insect-resistant transgenic poplar study for the last twenty years in many aspects,including the effect of different types of insect-resistant genes,toxic protein expression,gene polymerization,insect-resistant stability and biological security.Problems existing in the genetic transformation of multigenes were put forward,such as individual gene missing or gene silencing.Strategies on how to express efficiently and simultaneously of multigenes in plants were discussed,especially the method that should be improved in vector construction was proposed.更多还原