【摘要】 以低成本的泔水油和豆粕为碳、氮源,发酵培养不动杆菌菌株XH-2（Acinetobacter sp.XH-2）生产生物表面活性剂。以表面张力及排油圈直径为考察指标,对菌株XH-2产生物表面活性剂的发酵条件进行单因素优化,并研究了该生物表面活性剂的稳定性。结果表明,菌株XH-2在最优培养基（泔水油3%、豆粕5%、氯化镁2%、磷酸氢二钾6%、磷酸二氢钾3.8%、三氯化铁0.5%、氯化钠2%和初始pH值6.0）中发酵培养2d后,所产的生物表面活性剂的临界胶束浓度为200mg·L^-1,其使发酵液的表面张力由73.01mN·m^-1降至25.25mN·m^-1,具有广泛的温度、pH值和盐度适应性,初步的成分分析结果表明该生物表面活性剂可能为糖脂类。更多还原

【Abstract】 Using low-cost swill oil and soybean meal as carbon and nitrogen source,the biosurfactant was produced by fermentation with Acinetobacter sp.XH-2.The fermentation conditions were optimized by dropcollapse test and surface tension test.The stability of biosurfactant was also explored.Results showed that critical micelle concentration of biosurfactant produced by strain XH-2was 200mg·L^-1 after fermentation for 2d and the surface tension of the broth was reduced from 73.01mN·m^-1 to 25.25mN·m^-1 under the optimized fermentation conditions:swill oil 3%,soybean meal 5%,MgCl₂2%,K₂HPO₄6%,KH₂PO₄3.8%,FeCl₃0.5%,NaCl 2% and initial pH value 6.0.The biosurfactant was stable over wide temperature,pH value and salinity.Preliminary component analysis results indicated that the biosurfactant might belong to glycolipids.更多还原