【摘要】 目的探讨Reprimo基因3’非翻译区824位点G>C单核苷酸多态性改变与肺癌发生的关系。方法基因分型用Ta Ka Ra的Mighty Amp DNA聚合酶法,分别检测36例肺癌患者(癌症组)及其癌旁正常组织(对照组)的Reprimo基因3’非翻译区824位点G>C分布,运用χ2检验和Armitage’s趋势检验等分析其与肺癌发生的相关性。结果两组标本经Hardy-Weinberg平衡检测,对照组在Reprimo基因3’非翻译区824位点G>C基因型观测(理论)频数为34、1、1(33.06、2.88、0.06),差异有统计学意义(P=0.042),癌症组的基因型观测(理论)频数为33、1、2(33.17、4.56、0.17),差异有统计学意义(P=0.003);Reprimo基因3’非翻译区824位点G>C单核苷酸多态性与肺癌易感性的Armitage’s趋势检验差异无统计学意义(χ2=0.31,P=0.581)。结论对照组接近符合Hardy-Weinberg遗传平衡状态,而肺癌组不符合该规律;Reprimo基因3’非翻译区824位点G>C单核苷酸位点多态性可能与肺癌发生不具有相关性。更多还原

【Abstract】 Objective To investigate the relationship between 824 G>C polymorphism in the 3’-untranslated region of Reprimo gene and lung cancer. Methods Genotyping was performed by Mighty Amp DNA Polymerase(TaKa Ra) with samples of cancerous tissues(cancer group) and pericarcinous tissues(control group) from 36 lung cancer patients to detect the 824 G>C polymorphism in the 3’-untranslated region of Reprimo gene. χ2test and Armitage’s trend test were applied to analyze the correlation between 824 G>C polymorphism and the risk of lung cancer. Results According to Hardy-Weinberg equilibrium detection, 824 G>C genotype observation(theory) frequency was 34, 1, 1(33.06, 2.88, 0.06) in the control group(with statistically significant difference, P=0.042) and 33, 1, 2(31.17, 4.56, 0.17)in the cancer group(with statistically significant difference, P=0.003). Armitage’s trend test revealed no significant correlation between 824 loci G>C single nucleotide polymorphisms and susceptibility to lung cancer( χ2=0.31, P=0.581).Conclusion The control group was nearly accord with Hardy-Weinberg genetic equilibrium state, but lung cancer group was not. No association was found between 824 G>C polymorphism in the 3’-untranslated region of Reprimo gene and lung cancer.更多还原