【摘要】 【目的】以东方百合品种明星无菌叶为材料建立快繁体系,为其种球产业化生产提供技术支撑。【方法】以花器官培养获得的无菌小鳞片及叶片为材料,以添加不同浓度6-BA、2,4-D的MS培养基诱导不定芽及小鳞茎产生;以不同浓度的蔗糖诱导小鳞茎的膨大及根的产生;以珍珠岩、草炭、蛭石的混合基质作为小鳞茎移栽基质。【结果】用6-BA诱导无菌小鳞片产生不定芽时以1.5 mg/L为宜,诱导率为75.0%;用2,4-D诱导无菌叶片产生小鳞茎时以2.0mg/L为宜,诱导率为91.7%;添加70.0 g/L蔗糖的MS培养基适宜小鳞茎膨大及根的产生;带小鳞茎及根的组培苗在珍珠岩:草炭:蛭石=1:1:1的基质中移栽成活率最高,达100.0%。【结论】东方百合品种明星可利用无菌叶片建立无性快繁体系。更多还原

【Abstract】 【Objective】Propagation system established with sterile leaves of Oriental lily Love Story as material to provide technical reference for industrialized production of its seed-bulbs.【Method】Sterile scales and leaves from flower organs culture were used as material and MS was used as basic medium,adding different concentrations of 6-BA and2,4-D to induce adventitious buds and bulb-lets;using different concentrations of sugar to induce bulb-lets expansion and rooting;perlite,peat and vermiculite were mixed as the transplanted matrix of bulb-lets.【 Result 】MS+1.5 mg/L 6-BA was appropriate for inducing adventitious buds with sterile scales and induction rate was 75.0%;the suitable sterile leaves differentiation bulb-lets medium was MS+2.0 mg/L 2,4-D and induction rate was 91.7%;the MS medium added 70.0 g/L sugar was suitable to induce bulb-lets expansion and rooting;perlite:peat:venniculite =1:1:1 was the best transplanted matrix of plant-lets with bulb-lets and roots,transplanting survival rate got 100.0%in this matrix.【Conclusion】Oriental lily Love Story could be established asexually rapid propagation system by sterile leaf.更多还原