【目的】明确引起禾花鲤发生暴发性死亡征的病原菌及其耐药性,为有效防控禾花鲤暴发性死亡征提供科学依据。【方法】采用常规方法分离病原菌,以API 20NE生化鉴定与16S r RNA基因序列分析相结合的方法进行鉴定,人工回归感染试验确定分离菌株的致病性,K-B纸片扩散法测定病原菌对药物的敏感性。【结果】从广西全州县患暴发性死亡征禾花鲤的肝脏中分离获得1株优势菌株(TH5),API 20NE生化鉴定和16S r RNA基因序列分析结果显示,分离菌株TH5为简达气单胞菌(Aeromonas jandaei),与简达气单胞菌标准菌株ATCC 49568(NR 119040)和CDC0787-80(NR 037013)的相似度分别为99.9%和99.6%。人工回归感染试验结果表明,菌株TH5是导致禾花鲤暴发性死亡征的病原菌。该菌株对复方新诺明、环丙沙星、多粘菌素B等11种药物高度敏感,对青霉素G、复达欣、先锋霉素VI等8种药物已产生耐药性。【结论】引起广西全州县养殖禾花鲤暴发性死亡征的病原菌是简达气单胞菌,生产中可选用复方新诺明、环丙沙星、多粘菌素B等药物进行防治。
【英文摘要】
【Objective】In order to provide reference for effective prevention and control of fulminant death for Procypris merus,the present experiment was conducted to investigate pathogen causing fulminant death of P.merus and its drug-resistance.【Method】The pathogen was isolated by conventional method,then the pathogen was identified based on API 20 NE biochemical identification and 16 S r RNA gene sequence analysis.Moreover,the pathogenicity of pathogen was determined by artificial infection.The K-B agar diffusion ...
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