【摘要】 基于苦荞转录组数据,采用RT-PCR技术克隆到1条苦荞过敏原蛋白基因的cDNA序列TBW17,并采用生物信息学方法对其编码蛋白进行了结构分析和抗原表位分析。结果表明,TBW17含有1个480 bp的开放阅读框,编码159个氨基酸,其氨基酸序列与多种植物过敏原蛋白同源性达52.20%~97.48%,蛋白家族分类显示其归为过敏蛋白中常见的Bet v1家族。抗原表位预测分析表明,该编码蛋白的T细胞表位位于91~99位,而B细胞表位位于第9~17、45~53和59~66位。以上结果为进一步对苦荞过敏原蛋白的检测和致敏机制研究提供了一定的理论基础。更多还原

【Abstract】 In the present study, the cDNA sequence named TBW17 was successfully cloned using RT-PCR technique according to buckwheat florescence transcriptome data, and its structural analysis and antigen epitope analysis were predicted by bioinformatics methods. The results showed that open reading frame(ORF) of TBW17 was 480 bp in length, encoding 159 amino acids. Multiple alignment analysis indicated TBW17 shared 52.20%~97.48% amino acid sequence homology with the other allergic proteins in plant, and it was classified into Betv1 family as a common allergy protein. Epitope prediction showed that the T-cell epitopes of TBW17 were located at residues 91~99 aa, and the B-cell epitopes were located at residues 9~17 aa, 45~53 aa, and 59~66 aa. The above results laid a theoretical foundation for the further study on detection and sensitization mechanism of the allergen protein from buckwheat.更多还原