【摘要】 为揭示猪肌分化因子(myogenic differentiation 1,MyoD1)基因启动子区多态性,本试验分别以野猪×从江香猪二元杂交猪、杜×长×大外三元杂交猪及贵州宗地花猪为研究对象,采用DNA池和直接测序技术,筛选MyoD1基因5′UTR及部分第1外显子区SNP位点,利用生物信息学软件预测SNP位点对核心启动子区、CpG岛和转录因子结合位点的影响。结果表明,在MyoD1基因5′UTR及部分第1外显子区筛查到3个SNPs位点,分别为A-39G、T+150C和C+227G;生物信息学软件预测发现,A-39G位点附近出现重要转录因子结合位点消失和新位点生成;CpG Island searcher软件分析得到多态位点突变前后CpG岛大小及GC含量发生改变,据此推测猪MyoD1基因5′UTR区域的A-39G位点对调控启动子功能元件有重要影响。更多还原

【Abstract】 In order to reveal the polymorphism of MyoD1 gene promoter in pigs,three pig breeds(wild boar Xiang pigs,commercial crossbred pigs and Guizhou Zongdi Hua pigs)were selected as the experimental subjects.By constructing DNA pool and using the technology of direct sequencing to screen the single nucleotide polymorphism on MyoD1 gene promoter region in three groups.Variety bioinformatics softwares were used to predict the core region of the promoter,transcription factors binding sites and CpG island.The results showed that the experiment had totally found three SNPs in MyoD1 gene,among these SNPs,A-39 G was located in 5′UTR,T+150Cand C+227G were located in exon 1.It was identified that various new transcription factors binding sites emerged and some significant transcription factors binding sites disappeared at the region nearby A-39 Gsite.The software of CpG island searcher indicated that SNP mutation could change the range of CpG island and the content of GC.Therefore,A-39 Gsite locating MyoD1 gene 5′UTR had an important effect on regulating the functional element of promoter.更多还原