【摘要】 试验旨在挖掘、分析策勒黑羊卵巢组织microRNA(miRNA),为进一步研究特定miRNA参与策勒黑羊卵泡发育及激素分泌等相关生殖活动的机制奠定理论基础。从策勒黑羊卵巢组织中提取总RNA,分离、构建小片段RNA文库,进行Solexa测序和生物信息学分析。结果表明,共获得9 527 311条干净序列读数,小RNA序列长度主要分布在21~23nt;共鉴定出434个策勒黑羊卵巢组织表达的保守miRNA,其中表达量在100以上有167个;鉴定出57个候选miRNA,其中表达量在100以上有8个。测序成功获取了策勒黑羊卵巢组织miRNA序列及表达谱,卵巢组织miRNA表达丰富且表达量各异。更多还原

【Abstract】 With the purposes of providing basic information for further studies of Qira Black sheep reproduction activities such as folliculogenesis and hormone secretion,characterization of microRNA(miRNA)expression in Qira Black sheep ovary tissues was studied.In this study,the small RNA isolated from total RNA of Qira Black sheep ovary tissues were sequenced by Solexa and then bioinformatics analysis were performed.The results showed that 9 527 311 clean reads were obtained,and the 21-23 nt small RNA was the major RNA in the total sequences.434 ovary conservative miRNA and 57 new miRNA were identified.Among them,there were 167 conservative including 8new miRNA exceeded 100 in the expression levels.The study succeeded to construct the expression library of miRNA which were abundant and differentially expressed in Qira Black sheep ovary tissues.更多还原