【摘要】 目的:探讨载脂蛋白E(Apo E)的多态性与阿尔茨海默病(AD)的相关性。方法:选取老年AD患者75例为AD组,另选取同期体检的健康老年人86例为对照组,采用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)对2组的Apo E基因多态性进行鉴定,并分析其与AD患病的相关性。结果:AD组ε3/ε4基因型频率高于对照组,差异有统计学意义(χ2=4.27,P<0.05),ε3/ε3是最常见的基因型;AD组ε4等位基因频率高于对照组,差异有统计学意义(χ2=4.40,P<0.05),ε3是最常见的等位基因。相关危险度分析显示,Apo E的三个等位基因中Apo Eε4基因与AD有相关性(RR=3.76,P<0.05),Apoε2和Apoε3基因与AD无相关性(RR=0.64、0.32,P>0.05)。ε4基因为1或2个的患者AD患病率更高(χ2=3.87、4.62,P<0.05)。结论:Apo Eε4基因是AD患病的重要危险因素。更多还原

【Abstract】 Objective:To explore the correlation between the polymorphisms of apolipoprotein E(Apo E) and alzheimer’s disease(AD). Methods: Total 75 AD patients were enrolled as AD group, and 80 healthy old people were enrolled as control group. The polymorphisms of Apo E gene were identified with the method of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Its correlation with AD was analyzed. Results: ε3/ε4 genotype frequency in the AD group was higher than that in the control group, with significant difference(χ2=4.27, P<0.05). ε3/ε3 was the most common genotype. The frequency of ε4 alleles polymorphism in the AD group was higher than that in the control group, with significant difference(χ2=4.40, P<0.05). ε3 alleles polymorphism was the most common. Related risk analysis showed that ε4 gene were associated with AD(RR =3.76, P<0.05), while ε3 and ε2 were not associated with AD(RR=0.64, 0.32, P>0.05). The prevalence of AD with one or two ε4 gene was higher(χ2=3.87, 4.62, P<0.05). Conclusion:ε4 gene of the Apo E is an important risk factor for AD.更多还原