【摘要】 目的:通过观察管通方醇提物对食管癌EC9706细胞增殖、细胞周期的影响,揭示该方抑制食管癌EC9706生长的作用机制。方法:体外培养食管癌EC9706细胞,用管通方醇提物处理细胞,MTT法检测管通方醇提物对食管癌EC9706细胞增殖影响的量效关系;倒置显微镜下观察食管癌EC9706细胞形态学变化;PI单染标记流式细胞术检测醇提物对食管癌EC9706细胞周期的影响。结果:管通方醇提物均可不同程度地抑制食管癌EC9706细胞的增殖,其抑制率呈剂量依赖关系;管通方醇提物影响了细胞形态结构。管通方醇提物对EC9706细胞周期各时相均有影响。与对照组比较,管通方组G0/G1期细胞比率升高(P<0.05)。结论:管通方醇提物将食管癌EC9706细胞阻滞于G0/G1,阻滞细胞周期的进程,该方对食管癌EC9706细胞的增殖具有抑制作用。更多还原

【Abstract】 Objective: To reveal the mechanism of Guan Tong formula inhibiting EC9706 growth of esophageal cancer by observing the effects of alcohol extract from Guan Tong formula on cell proliferation and cell cycle of EC9706. Methods: EC9706 cells of esophageal cancer were cultivated in vitro, and they were processed by alcohol extract from Guan Tong formula, the dose-effect relationship between the effects of alcohol extract from Guan Tong formula on EC9706 cell proliferation were detected by MTT method; the morphological changes of EC9706 cell of esophageal cell were observed under the inverted microscope; the effects of alcohol extract on EC9706 cell cycle of esophageal cancer were detected by using PI single staining flow cytometry. Results: EC9706 cell proliferation of esophageal cancer was inhibited by alcohol extract from Guan Tong formula in varying degrees, its inhibiting rate was dose-effect relationship; alcohol extract from Guan Tong formula influenced cell morphological structure and all stages of EC9706 cell cycle. Compared with the control group, the cell rate of G0/G1 checkpoint in Guan Tong formula group elevated(P<0.05). Conclusion: Alcohol extract from Guan Tong formula arrests EC9706 cell of esophageal cancer in G0/G1 and induces cell differentiation, this formula has the function of inhibiting EC9706 cell proliferation of esophageal cancer.更多还原