【摘要】 固体支撑脂质双层膜能很好的模拟生物膜系统,固体衬底的表面结构和性质会影响双层膜的成膜过程、流动性、微区形成以及化学活性,进而影响蛋白的特性。本文,我们在两种衬底云母和二氧化硅表面,构建了包含神经节苷脂GM1的固体支撑脂质双层膜,用拉曼光谱研究了两种衬底表面脂质膜的分子构象。并且,在脂质膜表面加入淀粉样-beta蛋白,分析两种衬底上淀粉样-beta蛋白的构象差异。更多还原

【Abstract】 Supported planar lipid bilayers are artificial lipid bilayer membranes.Surface structures and properties of the solid substrates can affect the formation process,fluidity,two-dimensional structure and chemical activity of the SPBs,then affect properties of protein.Even on mica and SiO2 surfaces,which are flat and biologically inert,and most widely used as the substrates for the supported lipid bilayers,cause differences in the structure and properties of the supported membranes.In this paper,by performing Raman spectroscopy,we analyzed the conformation of SPBs at mica and SiO2.Futhermore,we added the monomer amyloid-beta peptides onto SPBs.The differences between the conformation of amyloid-beta at mica and SiO2 are shown by Raman spectroscopy with different incubation time.更多还原