【摘要】 目的了解不同食源性传播介质中产超广谱β-内酰胺酶(ESBLs)大肠埃希菌的分布情况及同源性。方法从2014—2015年韶关市不同来源的食源性样本中分离产ESBLs大肠埃希菌,采用脉冲场凝胶电泳(PFGE)技术和Bio Numerics软件对菌株进行分型。结果 347份不同来源的食源性样本中检出11株致腹泻大肠埃希菌和29株产ESBLs大肠埃希菌。PFGE图谱分析,29株菌可以分为21个聚类群,其中聚类A包含7株菌(J2、J3、J4、J7、J9、B4、S3),为优势的PFGE带型,聚类B有2株菌(J6、J8),其余菌株带型为分散克隆。3株健康从业人员分离菌(J2、J7、J9)相似系数为100%,生活饮用水分离菌(S3)与腹泻患者分离菌(B4)的相似系数为97.1%,S3菌与4株健康从业人员分离菌(J2、J3、J7、J9)相似系数均>90.0%。结论韶关市食源性产ESBLs大肠埃希菌在食品、水、动物和人群中广泛分布,并可能通过食物链相互传播,应加强监测,防止进一步扩散。更多还原

【Abstract】 Objective To study the distribution and homology of foodborne-associated extended-spectrum β-lactamases( ESBLs)-producing Escherichia coli( E. coli). Methods ESBLs-producing E. coli were isolated from different food specimens in Shaoguan from 2014 to 2015,strains were typed with pulsed-field gel electrophoresis( PFGE) and Bio Numerics software. Results 11 strains of diarrhea-causing E. coli and 29 strains of ESBLs-producing E. coli were isolated from 347 different sources of food specimens. PFGE analysis showed that 29 strains could be divided into 21 cluster groups,group A was predominant pattern,which included 7 strains( J2,J3,J4,J7,J9,B4,S3),group B included 2 strains( J6,J8),the other strains were sporadic clones. Similarity coefficient( SC) of 3 strains( J2,J7,J9) from health practitioners was100%,SC of strains from drinking water and patients with diarrhea( B4) was 97. 1%,SC of S3 strain and 4 strains( J2,J3,J7,J9) from health practitioners were all > 90. 0%. Conclusion Foodborne-associated ESBLs-producing E. coli are widely distributed in food,water,animal,and populations,and can be transmitted through food chain,surveillance should enhanced to avoid further spread.更多还原