The aim of this study is to identify the semiochemicals from Oncomelania hupensis. The extraction method was used to extract the soft tissues of Oncomelania hupensis,and the extraction time and extraction solvent were optimized. GC-MS method was used to qualitative and semi-quantitative analysis. The results showed that there were 12 substances in the male Oncomelania hupensis and 15 substances in the female Oncomelania hupensis respectively. The relatively high contents were sterols and esters.