【摘要】 目的研究Rho激酶通路对醛固酮（ALD）诱导的血管平滑肌细胞（VSMC）增殖的影响。方法用Western blot法检测ALD诱导的血管平滑肌细胞Rho激酶的表达,氚标记胸腺嘧啶掺入法检测ALD诱导的VSMC的增殖,并观察ALD受体MR在此过程中的作用。结果 ALD可剂量、时间依赖地上调VSMC Rho激酶的表达,最强的刺激时间为20 min,最强的刺激浓度为100nmol·L^-1,ALD受体拮抗剂——依普利酮可显著抑制ALD诱导的VSMC Rho激酶的上调及VSMC的增殖,依普利酮和Rho激酶抑制剂——Y-27632可显著逆转ALD的上述效应。结论 Rho激酶信号传导通路及ALD受体在ALD诱导的VSMC增殖中起重要作用。更多还原

【Abstract】 Objective To explore the role of Rho kinase in vascular smooth muscle cell（ VSMC） proliferation induced by aldosterone（ ALD）and the effect of mineralcocorticoid receptor（ MR）. Methods The expressions of Rho-kinase protein were detected by Western blot. VSMC proliferation was measured by [3H] Thymidine incorporation. Results ALD could up-regulate the expressions of Rho-kinase protein in dose-and time-dependent manner,peak at 20 min and 100 nmol·L^-1.Eplerenone,mineralcocorticoid receptor antagonist,could significantly reverse these effects,indicating the activation of Rho-kinase pathway induced by ALD in VSMC was mediated by MR. ALD could significantly induce VSMC proliferation,which could be inhibited by eplerenone and Y-276329（ Rho-kinase inhibitor）,indicating the VSMC proliferation induced by ALD was mediated by MR and the activation of Rho-kinase pathway. Conclusion Rho-kinase pathway and MR play an important role in VSMC proliferation induced by ALD.更多还原