【摘要】 利用静电纺丝技术制备了纳米银(n-Ag)/山莨菪碱(AD)/聚乳酸-乙醇酸(PLGA)功能性材料.通过场发射扫描电子显微镜(ESEM)和傅里叶变换红外光谱(FTIR)研究了材料的微观结构及组成,评价了材料的体外药物释放、抑菌性、细胞相容性及细胞毒性.FTIR结果表明,山莨菪碱已担载到n-Ag/AD/PLGA材料中,且随着山莨菪碱含量的增加,n-Ag/AD/PLGA材料内纤维的平均直径逐渐增大.药物释放试验结果证明,山莨菪碱可以逐渐释放.当山莨菪碱的质量分数为1%~5%时,n-Ag/AD/PLGA材料无毒性,且有助于小鼠L929成纤维细胞的生长和增殖.研究结果表明,n-Ag/AD/PLGA材料具有良好的抗菌性能和细胞相容性,担载的山莨菪碱可有效释放,为糖尿病足部感染的临床治疗提供新的人工敷料.更多还原

【Abstract】 Nano-silver powders( n-Ag) / Anisodamine( AD) / poly( lactide-co-glycolide)( PLGA) were prepared by electrospinning. Field emission scanning electron microscope( ESEM) and Fourier transform infrared spectroscopy( FTIR) were applied for the investigation of the microscopic structure of the dressings as well as drug release,antibacterial and biological functions so that we could comprehensively evaluate the functionality of the dressings. The results show that the fibers exhibit a fully interconnected pore structure with narrow pore size distribution. And the diameters of electrospun fibers are influenced by the incorporation of AD.The result of drug release test proves that anisodamine can gradually release. It is also found that the n-Ag /AD / PLGA dressings exhibit a well antibacterial ability. The cell experimental results show that when the content of AD is from 1% to 5%,the dressings are non-toxic and contribute to the growth and proliferation of mouse L929 cells. Therefore,we conclude that the n-Ag / AD / PLGA dressing possesses good biocompatibility,antibacterial properties,and effectively release. It provides a new strategy for clinical treatment in diabetic foot infection.更多还原