【摘要】 目的本文利用tau基因敲除小鼠模型,观察tau基因表达水平的变化对中脑部位Nurr1蛋白表达的影响。方法取同窝出生的同性别的野生型(Tau+/+)、杂合子(Tau+/-)、纯合子(Tau-/-)系列tau基因敲除小鼠。应用免疫印迹技术定量检测不同年龄3种基因型小鼠中脑部位Nurr1的表达水平;应用免疫荧光染色的方法,定位定量检测18月龄3种基因型小鼠中脑黑质致密部(SNC)和腹侧背盖区(VTA)Nurr1和TH的表达水平。结果免疫印迹的结果显示Nurr1在6月龄至24月龄Tau-/-小鼠中脑的表达量增多,与Tau+/+和Tau+/-小鼠相比差异有统计学意义(P<0.05);在免疫荧光实验中,Nurr1在Tau-/-小鼠中脑SNC和VTA部位TH阳性神经元中表达量增多,与Tau+/+和Tau+/-小鼠相比差异有统计学意义(P<0.05);3种基因型小鼠中脑部位TH表达量差异无统计学意义。结论 Nurr1在成年Tau基因敲除小鼠中脑多巴胺神经元中表达上调,而中脑TH表达水平无改变,Nurr1可能参与维持TH的表达。更多还原

【Abstract】 Object To anaylsis the expression of Nurr1 in tau-knockout mice model midbrain under different levels of tauexpression. Methods We conducted western bolt analysis to detect the expression of Nurr1 in the midbrain of tau-knock mice atthe age of 6, 12, 18 and 24 month, separately.(Including genotype Tau+/+, Tau+/- and Tau-/-). We measured the expression ofNurr1 and TH by using immunofluorescence technique in the SNC and VTA of tau-knock mice at 18-month-old. Results West-ern blot results showed that the expression of Nurr1 in the midbrain of 6-, 12-, 18- and 24- month-old Tau-/- mice was significant-ly upregulated(P<0.05). A significantly increase of nuclear Nurr1 expression was observed in TH+ neurons of 18-month-old Tau-/-mice compared with control Tau+/+ mice and Tau+/- mice(P<0.05) in the SNC and VTA. However, there was no significant differ-ence in the expression of TH in the midbrain. Conclusion The expression of Nurr1 in midbrain TH+ neurons of Tau-/- mice wassignificantly upregulated, while there was no variety in the expression of TH in the midbrain, which suggested that Nurr1 may main-tain the expression of TH.更多还原