【摘要】 采用MTT法测定配合物1~3对5种肿瘤细胞株(T-24、SK-OV-3、SK-OV-3/DDP、BEL-7402、HCT-8)和1种人正常肝细胞HL-7702进行体外活性测试,并利用琼脂糖凝胶电泳实验初步探讨了配合物1~3与p UC19 DNA相互作用。实验结果表明,相对于正常细胞HL-7702,配合物1~3对5种肿瘤细胞的生长均表现出不同程度的抑制作用,其IC50范围为5.7~40.1μM。同时,研究还发现配合物1~3以插入作用方式与DNA作用。更多还原

【Abstract】 The antitumor activity of quinoline-based silver(I) complexes 1~3 against T-24, SK-OV-3, SK-OV-3/DDP, BEL-7402, HCT-8 tumor cell lines and human normal liver cell line HL-7702 were screened by MTT assay. The binding mode of 1~3 with p UC19 plasmid DNA was examined by agarose gel electrophoresis assay. The results indicated 1~3 exhibit selective cytotoxicity against-24, SK-OV-3, SK-OV-3/DDP, BEL-7402, HCT-8 cell lines with IC50 values generally in the micromolar range(5.7~40.1 μM), but low cytotoxicity towards normal human liver cell HL-7702. The interactions of 1~3 with DNA are most probably via an intercalation.更多还原