【摘要】 PHR1是一种转录因子,在植物对磷的吸收过程中起重要作用,本研究为了获得高磷利用效率的小麦,采用基因枪法遗传转化了普通小麦品种矮抗58,获得转缺磷响应转录因子Ta PHR1基因小麦新品系T8,PCR和RT-PCR检测表明外源基因已经整合到小麦基因组并正确转录,为明确该转基因小麦的磷利用特性,以T8和其受体对照矮抗58为试验材料,研究了试材的地上部吸磷量和产量性状。结果表明,转Ta PHR1基因小麦新品系T8在CK、N、NK和NPK处理下的产量和地上部吸磷量均显著高于其受体对照矮抗58,其中在N处理下的产量可增加10.64%,吸磷量可增加12.29%;大田条件下转基因小麦单株产量和小区产量分别较其受体矮抗58增产10.49%和7.32%,千粒重的增加是T8增产的主要因素;转Ta PHR1基因小麦较对照具有更好的磷吸收特性和增产潜力。更多还原

【Abstract】 PHR1 is a transc ription factor, which plays an important role in plant phosphorus absorption. This study was to obtain higher phosphorus utilization efficiency wheat, we transferred Ta PHR1 gene into Aikang58 by particle bombardment transformation method, and obtained a transgenic line named T8. PCR and RT-PCR detection showed that the foreign gene had been integrated into the wheat genome and transcribed correctly. To identify phosphorus uptake characteristics of the transgenic wheat, we took T8 and Aikang58 as material, yield and phosphorus uptake characteristics were studied, the results showed that yield and shoot phosphorus uptake of T8 in CK, N, NK and NPK treatment were increased than wild type(Aikang58) respectively, the yield and shoot phosphorus uptake, under N treatment, were increased by 10.64% and 12.29% than Aikang58 respectively; The yield of T8 increased 10.49% and 7.32% than Aikang58 under single plant and plot field conditions respectively, the increase of 1 000-kernel weight was the main factor to increase the yield of T8. The results described above indicated that the transgenic wheat line expressing Ta PHR1 gene had better phosphorus absorption characteristics and yield potential than wild type.更多还原