【摘要】 本研究从103对SSR引物中筛选出40对稳定扩增的多态性SSR引物,多态性比率为39.1%,并对213份鲜食玉米自交系的亲缘关系进行分子评价。研究表明,40对SSR标记共扩增出169个等位基因,每个标记位点等位基因数变幅从2到9个,平均等位基因数为4.2。多态性信息含量(PIC)值范围从0.72到0.91,平均每对引物0.82。基于非加权组平均法(UPGMA)聚类分析将供试玉米自交系划分为3个大类群,第3类群又划分7个亚群,遗传聚类分析结果基本与自交系谱系及来源相符。更多还原

【Abstract】 In t his study, 40 polymorphic SSR primers were screened from 103 pairs of SSR primers, and their polymorphic rate was 39.1%. We did the molecular evaluation on the genetic relationships of 213 maize inbred lines. The study showed that 169 alleles were amplified with 40 SSR markers, and the number of alleles per locus varied from 2 to 9 with an average of 4.2 alleles. The value of Polymorphic Information Content(PIC) varied from0.72 to 0.91 with an average value of 0.82. Based on clustering analysis using UPGMA method(Unweighted Pair-Group Method with Arithmetic Means), maize inbred lines were classified into three major groups, of which the third group was divided into seven subgroups. The clustering result was consistent with the groups based on the inbred pedigree system and sources.更多还原