【摘要】 脱落酸(ABA)在植物生长发育和逆境胁迫响应等过程中具有重要作用。脱落酸8’-羟化酶是ABA分解及其代谢的关键酶,可降解内源ABA水平。本研究利用Reverse Transcription PCR(RT-PCR)的方法从花生品种闽花6号叶片中克隆了脱落酸8’-羟化酶基因AhCYP707A4的开放阅读框(Open Reading Frame,ORF)。AhCYP707A4基因ORF长1 446 base pairs(bp),编码481个氨基酸。生物信息学分析表明,该蛋白N端有一个长32个氨基酸残基的信号肽序列,其后为P450蛋白保守区,符合典型的P450蛋白的结构特征。氨基酸多序列比对显示AhCYP707A4具有较高的保守序列。实时荧光定量PCR分析表明,该基因在不同发育时期的果皮、种皮和胚中均有表达。在果皮和种皮中,该基因在花生果针入土后40 d(days after pegging,DAP)表达量最高,而胚中在60 d中表达量达到最高。AhCYP707A4基因可能在花生发育过程起重要的调控作用,是调控花生种子发育特别是胚发育的重要候选基因。更多还原

【Abstract】 Abscisic acid(ABA) plays important roles in plant growth and development as well as in stress responses.8’- hydroxylase is the key enzyme for ABA catabolism and it can degrade endogenous ABA levels in plants. In this study, we used RT-PCR method to obtain the full-length of AhCYP707A4 open reading frame(ORF) from Minhua6 peanut leaf. The length of AhCYP707A4 ORF was 1 446 bp encoding 481 predicted amino acids. Bioinformatics analysis showed that AhCYP707A4 has a signal peptide sequence with 32 amino acid residues at the N side,followed by P450 protein conservative area. This features conform to the structure characteristics of the typical P450 proteins. Multiple sequence alignment of CYP707A4 proteins revealed that the AhCYP707A4 has a high conservative in sequence. Real-time PCR analysis showed that AhCYP707A4 gene can express in the pericarp,testa and embryo at different stages. It has the highest expression level in pericarp and testa at 40 DAP. However,the relative expression level in embryo reached the highest at 60 DAP. AhCYP707A4 may play an important regulatory role in peanut development process and is an candidate gene related to peanut seed development especially embryo development.更多还原