【摘要】 为了对福建省发生的番茄黄化曲叶病毒病的病原进行分子鉴定,并对其病原分子的变异和进化情况进行分析。根据已知的番茄黄化曲叶病毒(TYLCV)基因组序列设计特异引物进行PCR扩增,对获得的特异性片段回收、克隆并测序,结果表明,PCR分子标记反应扩增到541bp的特异性条带,将该特异性产物经回收和纯化并进行克隆与测序,测序结果经BLAST比对后,表明该分离物的序列与国内外的病毒序列相似度为97%~99%。试验结果表明福建省的5个地市的番茄均已被番茄黄化曲叶病毒病危害,应当加强防治。更多还原

【Abstract】 Molecular identification,variation,and phylogeneticity of the yellow leaf curl virus(TYLCV)on tomato plants in Fujian were investigated.For the study,specific primers were designed according to the known genome sequence of TYLCV for PCR detection.Then,the generated specific segments were collected,cloned and sequenced.A 541 bp fragment was obtained and its nucleotide sequence showed a high homogeneity,i.e.,97%-99%,with that of the tomato yellow curl leaf virus isolated from the domestic as well as international origins.It confirmed the presence of TYLCV infection on tomato plants in 5cities in the province,and disease prevention and control measure should soon be implemented.更多还原