【摘要】 为探究光合细菌PB-Z产氢的最适产氢条件,在单因素实验的基础上,以镁离子浓度、p H、反应时间为实验因素,以光合细菌PB-Z产氢量为响应值,采用响应面分析法对实验结果进行模拟及分析,建立了产氢量与3个自变量关系的二次多项式数学模型。结果表明,镁离子浓度、p H值、反应时间与产氢量存在显著的相关性。确定光合细菌PB-Z的最佳产氢条件为:镁离子浓度4.77 mmol/L,初始p H 7.05,反应时间77.5 h。在此实验条件下,产氢量为689.3 m L/L-培养基,与预测值相比偏差仅4.8%。说明响应曲面法优化光合细菌PB-Z的产氢条件是可行的。更多还原

【Abstract】 The most suitable hydrogen production conditions of photosynthetic bacteria were studied for replication.On the basis of single factor experiment, magnesium ion concentrations, initial p H value, reaction time were selected as independent variables, and the hydrogen yield as the response value, experimental results were simulated and analyzed by response surface methodology. A quadratic polynomial equation was established for describing the relationship between hydrogen yield and three independent variables. Response surface analysis results demonstrate that the selected factors had significant correlation with the hydrogen yield. The optimum conditions of hydrogen yield were determined to be magnesium ion concentration 4.77mmol/L, initial p H value 7.05, reaction time 77.5 h. Under these conditions, the hydrogen production of photosynthetic bacteria was 689.3 ml/L-culture, the value agreed with the predicted value with 4.8% deviation. The results showed that the experimental value was comparable to model prediction and the method was practical.更多还原