【摘要】 盐胁迫是影响植物生长发育的非生物胁迫因素之一,目前发现的盐胁迫信号途径的基因还非常有限.发掘新的耐盐相关基因对于了解植物的盐胁迫响应机制和改善作物的耐盐性具有非常重要的意义.从EMS突变体库中筛选突变体是一种经典的正向遗传学方法,有可能筛选到从T-DNA插入突变体库中无法发现的新基因.本实验用160mmol/L NaCl作为盐胁迫的筛选条件,以在含盐平板上长出绿色子叶作为筛选指标筛选抗盐(Salt Resistant,ST)的EMS突变体,初步筛选到了140株ST突变体,其中84株收到了种子,第二代进行抗盐表型确定后,得到了15株表型稳定的ST突变体,均表现出明显的抗盐表型,并对其中的一些突变体做了初步的表型及基因定位分析.更多还原

【Abstract】 Salinity is one of the major abiotic stresses that affects the growth and development of plants.So far very limited salt-resistant genes have been cloned and studied.Identifying new genes involved in salt tolerance are very important for understanding the salt signaling pathway and improving salt tolerance of plants.EMS mutagenesis is a classic method of reverse genetics.It can be used to discover novel salt-resistant genes which may not be identified by screening T-DNA insertion lines.In this study,160mmol/L NaCl was used to screen for salt-tolerant mutants.The leaves of salt-tolerant mutants were green but that of the wild type plants turned yellow.140 strains of salt-tolerant mutants were identified initially,however,only 84 of them set seeds.After confirmation of the salt resistant phenotype using T2 generation,15strains of stable salt-tolerant mutants(ST mutants)were obtained.Genetic and phenotypic analysis and preliminary mutated gene mapping were done for some of the identified mutants.更多还原