【摘要】 为了观察植物多聚ADP核糖聚合酶[poly(ADP-ribose)polymerase,PARP]的酶活对植物生长的作用,利用PARP抑制剂来抑制拟南芥PARP的体内活性,并观察表型.体外生化实验结果表明,3-氨基苯甲酰胺(3-Aminobenzamide,3-AB)可以抑制拟南芥PARP1和PARP2的多聚ADP核糖化修饰活性;利用3-AB来处理拟南芥,发现抑制剂处理过的植物表现出更强的耐旱能力.为了排除3-AB的非特异性作用,使用PARP的另外一个抑制剂苯甲酰胺(Benzamide,BA)对拟南芥进行处理,结果显示,BA处理后拟南芥也表现出相似的抗旱表型.另外,在正常生长条件下,3-AB或BA处理过的拟南芥生长量较大,植株鲜重明显增加.以上结果说明,抑制拟南芥PARP活性可以促进拟南芥抗旱能力的改善,其抑制剂具有潜在的应用价值.更多还原

【Abstract】 In order to study the physiological role of poly(ADP-ribose)polymerase(PARP)in vivo,we used PARP inhibitors to suppress the activities of PARPs by adding the inhibitors into soil or the culture media.In vitro biochemical experiments showed that PARP inhibitor 3-AB can inhibit the catalysis activities of PARP1 and PARP2.Application of poly(ADP-ribose)polymerase inhibitor 3-AB improved the drought tolerance of Arabidopsis.The 3-AB treated seedlings retained more water in leaves and recovered better than control plants after drought stress.Another inhibitor BA also showed the similar effects as 3-AB.Under normal conditions,application of 3-AB or BA also increased the fresh weight of Arabidopsis.These results suggested that inhibition of PARP activities can improve Arabidopsis drought tolerance.更多还原