【摘要】 目的应用蛋白质组学方法比较和分析人宫颈上皮永生化细胞(H8细胞株)和宫颈癌细胞(Caski细胞株)核蛋白差异表达。方法蛋白质双向凝胶电泳(2-DE)和质谱筛选H8细胞和Caski细胞核蛋白的差异表达蛋白,Western blotting验证筛选蛋白TRA16。结果成功建立H8细胞和Caski细胞核蛋白双向电泳图谱,质谱鉴定出9种差异表达的蛋白质,其中有6种(TRA16、TIAF1、CDK6、PP4C、ZN211、ANR16)在Caski中高表达,有3种(INT6、MYST1、LMNA)在H8细胞中高表达,TRA16在Caski中表达高于H8细胞(P<0.05)。结论筛选出的H8细胞和Caski细胞差异表达的核蛋白为寻找新的宫颈癌及癌前病变标记物提供帮助。更多还原

【Abstract】 Objective To compare and analyze the nucleus proteins of human endocervical cells(H8)and cervical cancer cells(Caski)by two dimensional electrophoresis(2DE)and Mass Spectrometry(MS).Methods The differential expression nucleus proteins of H8 cells and Caski cells were selected by 2-DE and MS,and TRA16 was identified by Western blotting.Results Twodimensional proein maps of H8 cells and Caski cells were obtained.9notable differential nucleus protein spots were detected in 2-DE gels.Among the proteins identified,6proteins(TRA16,TIAF1,CDK6,PP4 C,ZN211,ANR16)were up-regulated in Caski cells,and 3proteins(INT6,MYST1,LMNA)were up-regulated in H8 cells.TRA16express better than H8 in caski cells(P<0.05).Conclusion There are differentially expressed proteins in H8 cells and Caski cells which may become potential and valuable diagnostic markers for cervical cancer and CIN.更多还原